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to check this prediction, we reintroduced chromosomal Lon expression under the Charge of a constitutive promoter within our variety strain, and recurring deep mutational scanning in biological triplicate (Supplementary file two). We seek advice from The 2 regimes as +Lon and –Lon variety. the standard of +Lon selection was similar to that of –Lon choice (Figure two—figure supplement one, Figure two—figure dietary supplement 2). Consistent with our speculation, the distribution of choice coefficients shifted towards much more unfavorable values while in the +Lon choice, depleting good selection coefficients and enriching for adverse or null coefficients (Figure 2A).

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